CoraLite® Plus 488-conjugated IL-17D Monoclonal antibody

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  FC experiment of human PBMCs using CL488-66143

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug CoraLite® Plus 488-conjugated IL-17D Monoclonal antibody (CL488-66143, Clone: 1D7H4) (red) or 0.25 ug CoraLite® Plus 488 Mouse IgG2b isotype control (11B8C4) (CL488-66360-3, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

IL-17D Monoclonal Antibody for FC (Intra)

Cat No. CL488-66143

Clone No.1D7H4

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC (Intra)

Synonyms

IL17D, interleukin 17D, IL-27, IL27, IL 27

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Tested Applications

• Positive FC (Intra) detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66143 targets IL-17D in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-17D fusion protein Ag6762
• Full Name: interleukin 17D
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC036243
• Gene Symbol: IL-17D
• Gene ID (NCBI): 53342
• RRID: AB_3084213
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8TAD2
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IL17D is a secreted cytokine with homology to the IL-17 family of proteins. IL17D is preferentially expressed in skeletal muscle, brain, adipose tissue, heart, lung, and pancreas. The treatment of endothelial cells with IL17D has been shown to stimulate the production of other cytokines including IL6, IL8 and CSF2/ GM-CSF. The increased expression of IL8 induced by IL17D was found to be NF-kappa B-dependent.