IL-17A Recombinant antibody

IL-17A Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230038B1

Cat no : 82905-1-RR

Synonyms

Il17a, Interleukin17A, interleukin 17A, Il17, IL 17A

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Validation Data Gallery

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  WB analysis of EL-4 using 82905-1-RR

  PMA, Ionomycin and Brefeldin A treated EL-4 cells were subjected to SDS PAGE followed by western blot with 82905-1-RR (IL-17A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 82905-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82905-1-RR against Mouse IL-17A were performed. The affinity constant is 7.59 nM.

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Tested Applications

• Positive WB detected in: PMA, Ionomycin and Brefeldin A treated EL-4 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82905-1-RR targets IL-17A in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 17A
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 14 kDa, 17 kDa
• GenBank Accession Number: NM-010552
• Gene Symbol: Il17a
• Gene ID (NCBI): 16171
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.