FC experiment of mouse peritoneal macrophages using CL647-65097
1X10^6 IFN-γ primed murine peritoneal macrophages stimulated with LPS were surface stained with PE Anti-Mouse CD11b (M1/70), then fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C), and intracellularly stained with 0.5 ug CoraLite® Plus 647 Anti-Mouse IL-12/IL-23 p40 (CL647-65097, Clone: C17.8) or 0.5 ug CoraLite® Plus 647 Rat IgG2a Isotype Control (2A3) (CL647-65209, Clone: 2A3).
1X10^6 IFN-γ primed murine peritoneal macrophages stimulated with LPS were surface stained with PE Anti-Mouse CD11b (M1/70), then fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C), and intracellularly stained with 0.5 ug CoraLite® Plus 647 Anti-Mouse IL-12/IL-23 p40 (CL647-65097, Clone: C17.8) or 0.5 ug CoraLite® Plus 647 Rat IgG2a Isotype Control (2A3) (CL647-65209, Clone: 2A3).
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
CL647-65097 targets IL-12/IL-23 p40 in FC (Intra) applications and shows reactivity with mouse samples.
FC experiment of mouse peritoneal macrophages using CL647-65097
1X10^6 IFN-γ primed murine peritoneal macrophages stimulated with LPS were surface stained with PE Anti-Mouse CD11b (M1/70), then fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C), and intracellularly stained with 0.5 ug CoraLite® Plus 647 Anti-Mouse IL-12/IL-23 p40 (CL647-65097, Clone: C17.8) or 0.5 ug CoraLite® Plus 647 Rat IgG2a Isotype Control (2A3) (CL647-65209, Clone: 2A3).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
IL-12/IL-23 p40 Monoclonal antibody
Catalog Number
CL647-65097
Citations
-
Dilutions
FC (INTRA) :
Applications
FC (Intra)
Reactivity
mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
