PerCP-Cyanine5.5 Anti-Mouse IL-12/IL-23 p40 Rabbit Recombinant Antibody

IL-12/IL-23 p40 Uni-rAb^TM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Conjugate

PerCP-Cyanine5.5 Fluorescent Dye

CloneNo.

230037C9

Cat no : CPY5-98004

Synonyms

Il12b, Il-12b, IL-12 p40, Il 12p40, Il 12b

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Validation Data Gallery

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  FC experiment of RAW 264.7 using CPY5-98004

  1X10^6 unstimulated or IFNγ and LPS treated (in the presence of protein transport inhibitors) RAW 264.7 cells were intracellularly stained with 0.25 ug PerCP-Cyanine5.5 Mouse IL-12/23 P40 Recombinant Antibody (CPY5-98004, Clone:230037C9), and 0.25 ug CoraLite® Plus 647 Anti-Mouse CD11b (M1/70) (CL647-65055, Clone: M1/70). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC (Intra) detected in: IFNγ RAW 264.7 cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CPY5-98004 targets IL-12/IL-23 p40 in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: IL-12/IL-23 p40
• Calculated Molecular Weight: 70 kDa
• Conjugate: PerCP-Cyanine5.5 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 482 nm / 690 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

IL-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).