IL-10 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

IL-10 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

8E4B6

Cat no : 60269-1-PBS

Synonyms

CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF

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Validation Data Gallery

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  WB analysis of Recombinant protein using 60269-1-Ig (same clone as 60269-1-PBS)

  Recombinant protein were subjected to SDS PAGE followed by western blot with 60269-1-Ig (IL-10 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60269-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 60269-1-Ig (same clone as 60269-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60269-1-Ig (IL-10 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60269-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 60269-1-Ig (same clone as 60269-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60269-1-Ig (IL-10 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60269-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MOLT-4 using 60269-1-Ig (same clone as 60269-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MOLT-4 cells using IL-10 antibody (60269-1-Ig, Clone: 8E4B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60269-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60269-1-PBS targets IL-10 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-10 fusion protein Ag14870
• Full Name: interleukin 10
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC104252
• Gene Symbol: IL-10
• Gene ID (NCBI): 3586
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.