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Mouse IL-1 beta Recombinant antibody, PBS Only

IL-1 beta Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241364D5

Cat no : 84136-5-PBS

Synonyms

IL 1beta, Il1b, IL-1,IL1, IL-1 beta,IL 1 beta,IL1beta, Il 1b

Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using IL-1 beta Recombinant antibody (84136-5-RR)

    WB analysis of THP-1 using 84136-5-RR (same clone as 84136-5-PBS)

    LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 84136-5-RR (IL-1 beta antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84136-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84136-5-RR

    Affinity and Kinetic Characterization of 84136-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84136-5-RR against Mouse IL-1 beta were performed. The affinity constant is 13.2 pM.

Product Information

84136-5-PBS targets IL-1 beta in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name interleukin 1 beta
Calculated Molecular Weight31kDa
Observed Molecular Weight31 kDa
GenBank Accession NumberNM_008361.4
Gene Symbol IL-1 beta
Gene ID (NCBI) 16176
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets.