IL-1 beta Recombinant antibody

IL-1 beta Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240902G6

Cat no : 82696-17-RR

Synonyms

IL1- beta, IL1 beta, IL 1 beta, IL 1, Catabolin

---

Validation Data Gallery

• 

  WB analysis using 82696-17-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82696-17-RR (IL-1 beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 82696-17-RR

  LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 82696-17-RR (IL-1 beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 82696-17-RR

  Biolayer interferometry (BLl) kinetic assays of 82696-17-RR against Human IL-1 beta were performed. The affinity constant is 60.2 nM.

---

Tested Applications

• Positive WB detected in: A431 cells, LPS and Brefeldin A treated THP-1 cells, HT-1080 cells, U-87 MG cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82696-17-RR is IL-1 beta Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interleukin 1, beta
• Calculated Molecular Weight: 269 aa, 31 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC008678
• Gene Symbol: IL1B
• Gene ID (NCBI): 3553
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to the activation of numerous downstream targets.