IL-1 beta Recombinant antibody, PBS Only

IL-1 beta Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240902G6

Cat no : 82696-17-PBS

Synonyms

IL1- beta, IL1 beta, IL 1 beta, IL 1, Catabolin

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Validation Data Gallery

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  WB analysis using 82696-17-RR (same clone as 82696-17-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82696-17-RR (IL-1 beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82696-17-PBS in a different storage buffer formulation.

• 

  WB analysis of THP-1 using 82696-17-RR (same clone as 82696-17-PBS)

  LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 82696-17-RR (IL-1 beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82696-17-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82696-17-RR

  Biolayer interferometry (BLl) kinetic assays of 82696-17-RR against Human IL-1 beta were performed. The affinity constant is 60.2 nM.

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Product Information

The immunogen of 82696-17-PBS is IL-1 beta Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interleukin 1, beta
• Calculated Molecular Weight: 269 aa, 31 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC008678
• Gene Symbol: IL1B
• Gene ID (NCBI): 3553
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to the activation of numerous downstream targets.