IL-1 Beta Monoclonal antibody, PBS Only (Detector)

• Featured Product
• KD/KO Validated

IL-1 Beta Monoclonal Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A1B4

Cat no : 66737-1-PBS

Synonyms

IL1- beta, IL1 beta, IL 1 beta, IL 1, Catabolin

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Validation Data Gallery

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  WB analysis of A431 using 66737-1-Ig (same clone as 66737-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66737-1-Ig (IL-1 Beta antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-87 MG using 66737-1-Ig (same clone as 66737-1-PBS)

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 66737-1-Ig (IL-1 Beta antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP80001-6

  Sandwich ELISA standard curve of MP80001-6, IL-1 beta Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 82696-8-RR. Detection antibody: HRP-conjugated 66737-1-PBS. Standard:HZ-1164. Range: 0.098-12.5 ng/mL.

• 

  IHC staining of human lung cancer using 66737-1-Ig (same clone as 66737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66737-1-Ig (same clone as 66737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 66737-1-Ig (same clone as 66737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 66737-1-Ig (same clone as 66737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 66737-1-Ig (same clone as 66737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 66737-1-Ig (same clone as 66737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

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Product Information

66737-1-PBS targets IL-1 Beta as part of a matched antibody pair:

MP80001-6: 82696-8-PBS capture and 66737-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-1 Beta fusion protein Ag26030
• Full Name: interleukin 1, beta
• Calculated Molecular Weight: 269 aa, 31 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC008678
• Gene Symbol: IL1B
• Gene ID (NCBI): 3553
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.