CoraLite®594-conjugated IKBKG Monoclonal antibody

IKBKG Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1F2E2

Cat no : CL594-66460

Synonyms

AMCBX1, FIP 3, FIP3, Fip3p, I kappa B kinase subunit gamma, IkB kinase subunit gamma, IKBKG, IKK gamma, IKKAP1, IKKG, IP, IP1, IP2, IPD2, NEMO, NF kappa B essential modifier, NF kappa B essential modulator

---

Validation Data Gallery

• 

  IF Staining of HeLa using CL594-66460

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®®594 IKBKG antibody (CL594-66460, Clone: 1F2E2 ) at dilution of 1:100.

---

Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66460 targets IKBKG in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IKBKG fusion protein Ag13358
• Full Name: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC012114
• Gene Symbol: IKBKG
• Gene ID (NCBI): 8517
• RRID: AB_2919997
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

IKBKG, also named as FIP3, NEMO, IKKAP1 and IKKG, is specifically phosphorylate serine or threonine residues that are followed by a proline residue. IKBKG is regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways.