IGSF2/CD101 Monoclonal antibody, PBS Only

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  WB analysis using 66333-1-Ig (same clone as 66333-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66333-1-Ig (IGSF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66333-1-PBS in a different storage buffer formulation.

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  WB analysis of NR8383 using 66333-1-Ig (same clone as 66333-1-PBS)

  NR8383 cells were subjected to SDS PAGE followed by western blot with 66333-1-Ig (IGSF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66333-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66333-1-Ig (same clone as 66333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66333-1-Ig (IGSF2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66333-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66333-1-Ig (same clone as 66333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66333-1-Ig (IGSF2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66333-1-PBS in a different storage buffer formulation.

IGSF2/CD101 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66333-1-PBS

Clone No.1E6A7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

IGSF2, Glu-Trp-Ile EWI motif-containing protein 101, EWI-101, EWI101, EWI 101

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Product Information

66333-1-PBS targets IGSF2/CD101 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IGSF2/CD101 fusion protein Ag23297
• Full Name: immunoglobulin superfamily, member 2
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC136484
• Gene Symbol: IGSF2
• Gene ID (NCBI): 9398
• RRID: AB_2881713
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q93033
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IGSF2, also known as CD101, is a low abundance protein. Expression of CD101 is found on monocytes, granulocytes and dendritic cells. In addition expression on T lymphocytes is important for cell activation. It plays a role as inhibitor of T-cells proliferation induced by CD3 and inhibits expression of IL2RA on activated T-cells and secretion of IL2.