IGFBP4 Recombinant antibody, PBS Only (Detector)

IGFBP4 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230543B11

Cat no : 83200-1-PBS

Synonyms

IBP 4, HT29 IGFBP, Deb2, BP4, BP 4

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00279-2

  Sandwich ELISA standard curve of MP00279-2, Human IGFBP4 Recombinant Matched Antibody Pair - PBS only. 83200-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0825. 83200-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

• 

  Sample test of MP00279-2

  A549 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The mean IGFBP4 concentration was determined to be 181.3 ng/mL in A549 cell supernatant.

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  Sample test of MP00279-2

  Serum of sixteen individual healthy human donors was measured. The IGFBP4 concentration of detected samples was determined to be 36.9 ng/mL with a range of 4.7 - 129.3 ng/mL.

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  Cytometric bead array standard curve of MP00279-1

  Cytometric bead array standard curve of MP00279-1, Human IGFBP4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83200-2-PBS. Detection antibody: 83200-1-PBS. Standard: Eg0825. Range: 1.56-50 ng/mL.

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Product Information

83200-1-PBS targets IGFBP4 as part of a matched antibody pair:

MP00279-1: 83200-2-PBS capture and 83200-1-PBS detection (validated in Cytometric bead array)

MP00279-2: 83200-4-PBS capture and 83200-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: insulin-like growth factor binding protein 4
• Calculated Molecular Weight: 28 kDa
• GenBank Accession Number: NM_001552.3
• Gene Symbol: IGFBP4
• Gene ID (NCBI): 3487
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.