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IGFBP4 Recombinant antibody, PBS Only (Capture)

IGFBP4 Uni-rAbTM Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230543H7

Cat no : 83200-4-PBS

Synonyms

IBP 4, HT29 IGFBP, Deb2, BP4, BP 4

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00279-2

    Sandwich ELISA standard curve of MP00279-2

    Sandwich ELISA standard curve of MP00279-2, Human IGFBP4 Recombinant Matched Antibody Pair - PBS only. 83200-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0825. 83200-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

  • Sample test of MP00279-2

    Sample test of MP00279-2

    A549 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The mean IGFBP4 concentration was determined to be 181.3 ng/mL in A549 cell supernatant.

  • Sample test of MP00279-2

    Sample test of MP00279-2

    Serum of sixteen individual healthy human donors was measured. The IGFBP4 concentration of detected samples was determined to be 36.9 ng/mL with a range of 4.7 - 129.3 ng/mL.

Product Information

83200-4-PBS targets IGFBP4 as part of a matched antibody pair:

MP00279-2: 83200-4-PBS capture and 83200-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name insulin-like growth factor binding protein 4
Calculated Molecular Weight28 kDa
GenBank Accession NumberNM_001552.3
Gene Symbol IGFBP4
Gene ID (NCBI) 3487
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.