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IGFBP2 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 78.1-5000 pg/mL

Cat No : MP00322-2

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00322-2, human IGFBP2 Recombinant Matched Antibody Pair - PBS only. 83283-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0823. 83283-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

    Sandwich ELISA standard curve of MP00322-2

    Sandwich ELISA standard curve of MP00322-2, human IGFBP2 Recombinant Matched Antibody Pair - PBS only. 83283-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0823. 83283-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Serum of eight individual healthy human donors was measured. The IGFBP2 concentration of detected samples was determined to be 109.5 ng/mL with a range of 47.4 - 199.1 ng/mL.

    Sample test of MP00322-2

    Serum of eight individual healthy human donors was measured. The IGFBP2 concentration of detected samples was determined to be 109.5 ng/mL with a range of 47.4 - 199.1 ng/mL.

  • Urine of eight individual healthy human donors was measured. The IGFBP2 concentration of detected samples was determined to be 6251.0 pg/mL with a range of 306.2 - 19423.8 pg/mL.

    Sample test of MP00322-2

    Urine of eight individual healthy human donors was measured. The IGFBP2 concentration of detected samples was determined to be 6251.0 pg/mL with a range of 306.2 - 19423.8 pg/mL.

  • MCF-7 human breast cancer cells (5 x 10^6 cells/mL) were cultured in DMEM and 10% fetal bovine serum, 4 mM L-glutamine, 4500 mg/L glucose, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatants was removed, assayed for human IGFBP2, and measured 3750.9 pg/mL. MDA-MB-453 human breast carcinoma cells (5 x 10^6 cells/mL) were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatants was removed, assayed for human IGFBP2, and measured 9611.6 pg/mL.

    Sample test of MP00322-2

    MCF-7 human breast cancer cells (5 x 10^6 cells/mL) were cultured in DMEM and 10% fetal bovine serum, 4 mM L-glutamine, 4500 mg/L glucose, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatants was removed, assayed for human IGFBP2, and measured 3750.9 pg/mL. MDA-MB-453 human breast carcinoma cells (5 x 10^6 cells/mL) were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatants was removed, assayed for human IGFBP2, and measured 9611.6 pg/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00322-2 targets IGFBP2 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 78.1-5000 pg/mL (Sandwich ELISA);

Capture antibody: IGFBP2 Recombinant antibody, PBS Only (Capture) 83283-3-PBS (240227D4). 100 μg. Concentration 1 mgl/ml.

Detection antibody: IGFBP2 Recombinant antibody, PBS Only (Capture/Detector) 83283-2-PBS (240227G3). 100 μg. Concentration 1 mgl/ml.

Alternative IGFBP2 matched antibody pairs: MP00322-1

Matched pair protein standard: Eg0823

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83283-3-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name IGF binding protein 2
Synonyms IBP-2, IBP2, IBP 2, BP2, BP 2
GenBank accession numberNM_000597.3
Gene symbol IGFBP2
UniprotP18065
Gene ID (NCBI) 3485
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83283-2-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name IGF binding protein 2
Synonyms IBP-2, IBP2, IBP 2, BP2, BP 2
GenBank accession numberNM_000597.3
Gene symbol IGFBP2
UniprotP18065
Gene ID (NCBI) 3485
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.