IGF2BP2 Recombinant antibody, PBS Only

IGF2BP2 Uni-rAb^TM Recombinant Antibody for WB, IP, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IP, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6L8

Cat no : 82757-2-PBS

Synonyms

insulin like growth factor 2 mRNA binding protein 2, IMP-2, IMP2, IGF-II mRNA-binding protein 2, IGF2 mRNA-binding protein 2

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Validation Data Gallery

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  WB analysis using 82757-2-RR (same clone as 82757-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82757-2-RR (IGF2BP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82757-2-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 82757-2-RR (same clone as 82757-2-PBS)

  IP result of anti-IGF2BP2 (IP:82757-2-RR, 4ug; Detection:82757-2-RR 1:4000) with HEK-293 cells lysate 1480 ug. This data was developed using the same antibody clone with 82757-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 82757-2-RR (same clone as 82757-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82757-2-RR (IGF2BP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82757-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 82757-2-RR (same clone as 82757-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82757-2-RR (IGF2BP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82757-2-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 82757-2-RR (same clone as 82757-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 82757-2-RR (IGF2BP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82757-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 82757-2-RR (same clone as 82757-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 82757-2-RR (IGF2BP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82757-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82757-2-PBS

  Biolayer interferometry (BLl) kinetic assays of 82757-2-RR against Human IGF2BP2 were performed. The affinity constant is below 1 pM.

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Product Information

82757-2-PBS targets IGF2BP2 in WB, IP, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IGF2BP2 fusion protein Ag2142
• Full Name: insulin-like growth factor 2 mRNA binding protein 2
• Calculated Molecular Weight: 594 aa, 65 kDa
• Observed Molecular Weight: 55-65 kDa
• GenBank Accession Number: BC021290
• Gene Symbol: IGF2BP2
• Gene ID (NCBI): 10644
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Insulin-like growth factor 2 mRNA-binding protein 2(IGF2BP2), ia a member of a family of mRNA binding protein, which can bind to several mammalian cells' mRNAs. Thus it may involve in the regulation of translation of mRNA. IGF2BP2's polymorphisms is risk factor for developing type 2 diabetes. This antibody raise against part of N-terminus of human IGF2BP2. IGF2BP2 has some isoforms with the MW of 54-66 kDa. Some literature has reported that multiple bands can be detected (PMID: 22427968, 36322753, 23069990).