PE Anti-Human IGF1R/CD221 Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood granulocytes using PE-98144

  1x10^6 human peripheral blood granulocytes were surface stained with 5 ul PE Anti-Human IGF1R/CD221 Rabbit Recombinant Antibody (PE-98144, Clone:241679A6) (red) or PE Rabbit IgG Isotype Control Recombinant Antibody (PE-98136, Clone: 240953C9) (blue). Cells were not fixed.

IGF1R/CD221 Recombinant Antibody for FC

Cat No. PE-98144

Clone No.241679A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

IGF 1 receptor, IGF1R, IGF-I receptor, IGFIR, IGFR

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Tested Applications

• Positive FC detected in: human peripheral blood granulocytes

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-98144 targets IGF1R/CD221 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: IGF I receptor
• Calculated Molecular Weight: 1367 aa, 155 kDa
• GenBank Accession Number: BC113612
• Gene Symbol: IGF1R
• Gene ID (NCBI): 3480
• RRID: AB_3674028
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08069
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

IGF1R, also named as CD221, is a receptor tyrosine kinase which mediates actions of INS-like growth factor 1 (IGF1). IGF1R binds IGF1 with high affinity and IGF2 and INS with a lower affinity, the activated IGF1R is involved in cell growth and survival control. It is crucial for tumor transformation and survival of malignant cells. IGF1R is a heterotetrameric receptor, composed of two alpha subunits (130 kDa each) and two beta subunits (~98 kDa each) (PMID: 6279656). Both the alpha and beta subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds.