IFT88 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

IFT88 Monoclonal Antibody for WB, IP, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4A4G5

Cat no : 60227-1-PBS

Synonyms

hTg737, IFT88, TG737, TPR repeat protein 10, TTC10

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Validation Data Gallery

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  WB analysis of human testis using 60227-1-Ig (same clone as 60227-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 60227-1-Ig (IFT88 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 60227-1-Ig (same clone as 60227-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 60227-1-Ig (IFT88 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

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  WB analysis of NCCIT using 60227-1-Ig (same clone as 60227-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 60227-1-Ig (IFT88 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

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  IP experiment of PC-3 using 60227-1-Ig (same clone as 60227-1-PBS)

  IP result of anti-IFT88 (IP:60227-1-Ig, 5ug; Detection:60227-1-Ig 1:800) with PC-3 cells lysate 1040ug. This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60227-1-Ig (same clone as 60227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60227-1-Ig (same clone as 60227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 60227-1-Ig (same clone as 60227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 60227-1-Ig (same clone as 60227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 60227-1-Ig (same clone as 60227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 60227-1-Ig (same clone as 60227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

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Product Information

60227-1-PBS targets IFT88 in WB, IP, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IFT88 fusion protein Ag4980
• Full Name: intraflagellar transport 88 homolog (Chlamydomonas)
• Calculated Molecular Weight: 94 kDa
• Observed Molecular Weight: 88-95 kDa
• GenBank Accession Number: BC030776
• Gene Symbol: IFT88
• Gene ID (NCBI): 8100
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926). This antibody was raised against the C-terminal region of human IFT88 and can detect the endogenous level of IFT88.