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IFN-gamma Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.625-80 ng/mL

Sandwich ELISA: 7.80-500 pg/mL

Cat No : MP01131-1

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP01131-1, Human IFN-gamma Recombinant Matched Antibody Pair - PBS only. 84246-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0004. 84246-2-PBS was HRP conjugated as the detection antibody. Range: 7.80-500 pg/mL.

    Sandwich ELISA standard curve of MP01131-1

    Sandwich ELISA standard curve of MP01131-1, Human IFN-gamma Recombinant Matched Antibody Pair - PBS only. 84246-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0004. 84246-2-PBS was HRP conjugated as the detection antibody. Range: 7.80-500 pg/mL.

  • Serum of eight individual healthy human donors was measured. Six measured less than the lowest human IFN-gamma standard, 7.8 pg/mL. Two samples read 24.0 pg/mL and 31.4 pg/mL.

    Sample test of MP01131-1

    Serum of eight individual healthy human donors was measured. Six measured less than the lowest human IFN-gamma standard, 7.8 pg/mL. Two samples read 24.0 pg/mL and 31.4 pg/mL.

  • Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. For the PBMC supernatant cultured for 3 days, the mean IFN-gamma concentration was determined to be 6.1 pg/mL in unstimulated PBMC supernatant, 65860.8 pg/mL in PHA stimulated PBMC supernatant.

    Sample test of MP01131-1

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. For the PBMC supernatant cultured for 3 days, the mean IFN-gamma concentration was determined to be 6.1 pg/mL in unstimulated PBMC supernatant, 65860.8 pg/mL in PHA stimulated PBMC supernatant.

  • Cytometric bead array standard curve of MP01131-1, IFNG/IFN-gamma Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84246-3-PBS. Detection antibody: 84246-2-PBS. Standard:Eg0004. Range: 0.625-80 ng/mL.

    Cytometric bead array standard curve of MP01131-1

    Cytometric bead array standard curve of MP01131-1, IFNG/IFN-gamma Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84246-3-PBS. Detection antibody: 84246-2-PBS. Standard:Eg0004. Range: 0.625-80 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP01131-1 targets IFN-gamma in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.625-80 ng/mL (Cytometric Bead Array); 7.80-500 pg/mL (Sandwich ELISA);

Capture antibody: IFN-gamma Recombinant antibody, PBS Only (Capture) 84246-3-PBS (241390F9). 100 μg. Concentration 1 mgl/ml.

Detection antibody: IFN-gamma Recombinant antibody, PBS Only (Detector) 84246-2-PBS (241390B1). 100 μg. Concentration 1 mgl/ml.

Alternative IFN-gamma matched antibody pairs: MP00498-1, MP00498-2, MP01131-2, MP50092-1

Matched pair protein standard: Eg0004

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 84246-3-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name IFN gamma
Synonyms IFNG, IFN-gamma,IFN gamma,IFN γ,IFNγ, IFN γ, IFN gamma, IFG
GenBank accession numberBC070256
Gene symbol IFNG
UniprotP01579
Gene ID (NCBI) 3458
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 84246-2-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name IFN gamma
Synonyms IFNG, IFN-gamma,IFN gamma,IFN γ,IFNγ, IFN γ, IFN gamma, IFG
GenBank accession numberBC070256
Gene symbol IFNG
UniprotP01579
Gene ID (NCBI) 3458
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.