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IFN-gamma Recombinant antibody, PBS Only (Detector)

IFN-gamma Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241390B1

Cat no : 84246-2-PBS

Synonyms

IFNG, IFN-gamma,IFN gamma,IFN γ,IFNγ, IFN γ, IFN gamma, IFG

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP01131-1

    Sandwich ELISA standard curve of MP01131-1

    Sandwich ELISA standard curve of MP01131-1, Human IFN-gamma Recombinant Matched Antibody Pair - PBS only. 84246-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0004. 84246-2-PBS was HRP conjugated as the detection antibody. Range: 7.80-500 pg/mL.

  • Sample test of MP01131-1

    Sample test of MP01131-1

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. For the PBMC supernatant cultured for 3 days, the mean IFN-gamma concentration was determined to be 6.1 pg/mL in unstimulated PBMC supernatant, 65860.8 pg/mL in PHA stimulated PBMC supernatant.

  • Sample test of MP01131-1

    Sample test of MP01131-1

    Serum of eight individual healthy human donors was measured. Six measured less than the lowest human IFN-gamma standard, 7.8 pg/mL. Two samples read 24.0 pg/mL and 31.4 pg/mL.

  • Cytometric bead array standard curve of MP01131-1

    Cytometric bead array standard curve of MP01131-1

    Cytometric bead array standard curve of MP01131-1, IFNG/IFN-gamma Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84246-3-PBS. Detection antibody: 84246-2-PBS. Standard:Eg0004. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP01131-2

    Cytometric bead array standard curve of MP01131-2

    Cytometric bead array standard curve of MP01131-2, IFNG/IFN-gamma Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84246-1-PBS. Detection antibody: 84246-2-PBS. Standard: Eg0004. Range: 0.625-40 ng/mL.

Product Information

84246-2-PBS targets IFN-gamma as part of a matched antibody pair:

MP01131-1: 84246-3-PBS capture and 84246-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01131-2: 84246-1-PBS capture and 84246-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name IFN gamma
Calculated Molecular Weight 166 aa, 19 kDa
GenBank Accession NumberBC070256
Gene Symbol IFNG
Gene ID (NCBI) 3458
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.