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CoraLite® Plus 488 Anti-Mouse IFN gamma (XMG1.2)

IFN gamma Monoclonal Antibody for FC (Intra)

Host / Isotype

Rat / IgG1, kappa

Reactivity

Mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

XMG1.2

Cat no : CL488-65153

Synonyms

Ifg, IFN g, IFN gamma, IFN γ, Ifng, IFN-gamma, IFNγ, IFN-γ, interferon gamma

Validation Data Gallery

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  • Flow cytometry (FC) experiment of actived mouse splenocytes T cell using CoraLite® Plus 488 Anti-Mouse IFN gamma (XMG1.2) (CL488-65153)

    FC experiment of actived mouse splenocytes T cell using CL488-65153

    1X10^6 unstimulated or PMA and Ionomycin stimulated (in the presence of Brefeldin A) C57BL/6 mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD4 (CL647-65104, Clone: GK1.5) and then fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Cells were then stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse IFN gamma (CL488-65153, Clone: XMG1.2).

Tested Applications

Positive FC (Intra) detected inmouse splenocytes
Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65153 targets IFN gamma in FC (Intra) applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Purified Recombinant Mouse IFN gamma
Full Name interferon gamma
Calculated Molecular Weight17 kDa
GenBank Accession NumberBC119063
Gene Symbol IFN gamma
Gene ID (NCBI) 15978
RRIDAB_2919269
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins. (PMID: 19268625; 10688427)

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 IFN gamma antibody CL488-65153Download protocol
Standard Protocols
Click here to view our Standard Protocols