CoraLite® Plus 488 Anti-Mouse IFN-gamma (XMG1.2)

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  FC experiment of actived mouse splenocytes T cell using CL488-65153

  1X10^6 unstimulated or PMA and Ionomycin stimulated (in the presence of Brefeldin A) C57BL/6 mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD4 (CL647-65104, Clone: GK1.5) and then fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. Cells were then stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse IFN gamma (CL488-65153, Clone: XMG1.2).

IFN-gamma Monoclonal Antibody for FC (Intra)

Cat No. CL488-65153

Clone No.XMG1.2

Host / Isotype

Rat / IgG1, kappa

Reactivity

mouse

Applications

FC (Intra)

Synonyms

IFN gamma, Ifng, Ifg, IFN g, IFN γ

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Tested Applications

• Positive FC (Intra) detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65153 targets IFN-gamma in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Purified Recombinant Mouse IFN gamma

• Full Name: interferon gamma
• Calculated Molecular Weight: 17 kDa
• GenBank Accession Number: BC119063
• Gene Symbol: IFN gamma
• Gene ID (NCBI): 15978
• RRID: AB_2919269
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P01580
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins. (PMID: 19268625; 10688427)