IFN-gamma Polyclonal antibody
IFN-gamma Polyclonal Antibody for IHC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
rat
Applications
IHC, FC (Intra), ELISA
Conjugate
Unconjugated
Cat no : 30293-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | rat spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC (Intra) detected in | PMA and ionomycin treated rat splenocytes cells |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
30293-1-AP targets IFN-gamma in IHC, FC (Intra), ELISA applications and shows reactivity with rat samples.
Tested Reactivity | rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | fusion protein |
Full Name | interferon gamma |
Calculated Molecular Weight | 18 kDa |
GenBank Accession Number | NM_138880 |
Gene Symbol | IFN-gamma |
Gene ID (NCBI) | 25712 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IFN gamma (IFNG) is a soluble cytokine that is the only member of the type II class of IFN. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB).
Protocols
Product Specific Protocols | |
---|---|
IHC protocol for IFN-gamma antibody 30293-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Surgery Sodium-glucose co-transporter 2 inhibitor canagliflozin modulates myocardial metabolism and inflammation in a swine model for chronic myocardial ischemia |