IFN-gamma Polyclonal antibody

IFN-gamma Polyclonal Antibody for IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

IHC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 30293-1-AP

Synonyms

IFN Gamma, Ifng, interferon gamma, IFN-γ, IFN-gamma,IFN gamma,IFN γ,IFNγ



Tested Applications

Positive IHC detected inrat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inPMA and ionomycin treated rat splenocytes cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30293-1-AP targets IFN-gamma in IHC, FC (Intra), ELISA applications and shows reactivity with rat samples.

Tested Reactivity rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen fusion protein
Full Name interferon gamma
Calculated Molecular Weight18 kDa
GenBank Accession NumberNM_138880
Gene Symbol IFN-gamma
Gene ID (NCBI) 25712
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFN gamma (IFNG) is a soluble cytokine that is the only member of the type II class of IFN. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB).

Protocols

Product Specific Protocols
IHC protocol for IFN-gamma antibody 30293-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Surgery

Sodium-glucose co-transporter 2 inhibitor canagliflozin modulates myocardial metabolism and inflammation in a swine model for chronic myocardial ischemia

Authors - Dwight D Harris