IFITM2-Specific Monoclonal antibody, PBS Only

• 

  WB analysis of HepG2 using 66137-1-Ig (same clone as 66137-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66137-1-Ig (IFITM2-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66137-1-PBS in a different storage buffer formulation.

• 

  WB analysis of recombinant protein using 66137-1-Ig (same clone as 66137-1-PBS)

  IFITM1 (Lane 1), IFITM2 (Lane 2) and IFITM3 (Lane 3) fusion proteins (20ng/lane) were subjected to SDS-PAGE followed by western blot with IFITM2 monoclonal antibody (66137-1-Ig) at dilution of 1:10,000. This data was developed using the same antibody clone with 66137-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66137-1-Ig (same clone as 66137-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66137-1-Ig (IFITM2-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66137-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 66137-1-Ig (same clone as 66137-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 66137-1-Ig (IFITM2-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66137-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

IFITM2-Specific Monoclonal Antibody for WB, Indirect ELISA

Cat No. 66137-1-PBS

Clone No.3D5F7

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

IFITM2, IFITM 2, DSPA2c, 3D5F7, 18D

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Product Information

66137-1-PBS targets IFITM2-Specific in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interferon induced transmembrane protein 2 (1-8D)
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: NM_006435
• Gene Symbol: IFITM2
• Gene ID (NCBI): 10581
• RRID: AB_2881536
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q01629
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death.(PMID:19544527) This antibody is specific to IFTIM2.