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IFITM2-specific Monoclonal antibody
IFITM2-specific Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
3D5F7
Cat no : 66137-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, recombinant protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 7 publications below |
| WB | See 20 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
The immunogen of 66137-1-Ig is IFITM2-specific Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Fusion Protein |
| Full Name | interferon induced transmembrane protein 2 (1-8D) |
| Observed Molecular Weight | 15 kDa |
| GenBank Accession Number | NM_006435 |
| Gene Symbol | IFITM2 |
| Gene ID (NCBI) | 10581 |
| RRID | AB_2881536 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death.(PMID:19544527) This antibody is specific to IFTIM2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IFITM2-specific antibody 66137-1-Ig | Download protocol |
| FC protocol for IFITM2-specific antibody 66137-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO J Zika virus induces massive cytoplasmic vacuolization and paraptosis-like death in infected cells. | ||
Proc Natl Acad Sci U S A mTOR inhibitors lower an intrinsic barrier to virus infection mediated by IFITM3. | ||
Mol Cell Proteomics HIV-1 Nef changes the proteome of T cells extracellular vesicles depleting IFITMs and other antiviral factors | ||
PLoS Pathog p53 promotes ZDHHC1-mediated IFITM3 palmitoylation to inhibit Japanese encephalitis virus replication.
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Claudia (Verified Customer) (06-20-2022) | The antibody worked very well for the analysis of IFITM2 expression in permanent cell lines.
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