IFIH1/MDA5 Monoclonal antibody

IFIH1/MDA5 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

3D8B11

Cat no : 66770-1-Ig

Synonyms

Helicard, Helicase with 2 CARD domains, Hlcd, IDDM19, IFIH1, IFIH1/MDA5, MDA 5, MDA5, RH116

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Validation Data Gallery

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  WB analysis using 66770-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66770-1-Ig (IFIH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66770-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66770-1-Ig (IFIH1/MDA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66770-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66770-1-Ig (IFIH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66770-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66770-1-Ig (IFIH1/MDA5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66770-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66770-1-Ig (IFIH1/MDA5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66770-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66770-1-Ig (IFIH1/MDA5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66770-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66770-1-Ig (IFIH1/MDA5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, THP-1 cells, Jurkat cells, Daudi cells, Raji cells
• Positive IHC detected in: human liver cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:8000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 4 publications below

Product Information

66770-1-Ig targets IFIH1/MDA5 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IFIH1/MDA5 fusion protein Ag17448
• Full Name: interferon induced with helicase C domain 1
• Calculated molecular weight: 1025 aa, 117 kDa
• Observed molecular weight: 117-125 kDa
• GenBank accession number: BC111750
• Gene symbol: IFIH1
• Gene ID (NCBI): 64135
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. IFIH1 has an apparent molecular mass of 117-130 kDa, and always other bands (70 kDa and 90 kDa) can be detected as cleaved products (PMID: 17267501).

Publications

Species	Application	Title
human	WB	J Virol; Encephalomyocarditis virus abrogates the IFN-β signaling pathway via its structural protein VP2.; Authors - Yumei Han
human	WB	Vet Microbiol; DDX56 antagonizes IFN-β production to enhance EMCV replication by inhibiting IRF3 nuclear translocation; Authors - Shujuan Xu
human	WB	Virus Res; Host protein, HSP90β, antagonizes IFN-β signaling pathway and facilitates the proliferation of encephalomyocarditis virus in vitro.; Authors - Qian Li
pig	WB	Front Immunol; Temporally integrated transcriptome analysis reveals ASFV pathology and host response dynamics; Authors - Lin Lv