IFI35 Recombinant antibody

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  IF Staining of HeLa using 83160-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IFI35 antibody (83160-2-RR, Clone: 240063C6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of A549 using 83160-2-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Ifi35 Recombinant Antibody (83160-2-RR, Clone:240063C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

• 

  FC experiment of HeLa using 83160-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ifi35 Recombinant Antibody (83160-2-RR, Clone:240063C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

• 

  Affinity and Kinetic Characterization of 83160-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83160-2-RR against Human IFI35 were performed. The affinity constant is below 1 pM.

IFI35 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Cat No. 83160-2-RR

Clone No.240063C6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

interferon induced protein 35, IFP35, IFP 35, Ifi-35, Ifi 35

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: A549 cells, HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83160-2-RR targets IFI35 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IFI35 fusion protein Ag34653
• Full Name: interferon-induced protein 35
• Calculated Molecular Weight: 31 kDa
• GenBank Accession Number: BC001356
• Gene Symbol: IFI35
• Gene ID (NCBI): 3430
• RRID: AB_3670853
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P80217
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFI35 (interferon induced protein 35), also known as IFP35. It is expected to be located in cytoplasm, nucleus and extracellular space. The protein is expressed in a wide range of cell types, including fibroblasts, macrophages, and epithelial cells. Involved in several processes, including macrophage activation involved in immune response; positive regulation of defense response; and regulation of signal transduction. And the calculated molecular weight of IFI35 is 31 kDa.