CoraLite® Plus 488-conjugated IFI16 Monoclonal antibody

IFI16 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C10D7

Cat no : CL488-67790

Synonyms

Ifi 16, IFI16, IFNGIP1, PYHIN2

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Validation Data Gallery

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  IF Staining of HeLa using CL488-67790

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 IFI16 antibody (CL488-67790, Clone: 1C10D7 ) at dilution of 1:400.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67790 targets IFI16 in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IFI16 fusion protein Ag30314
• Full Name: interferon, gamma-inducible protein 16
• Calculated Molecular Weight: 729 aa, 82 kDa
• Observed Molecular Weight: 85-95 kDa
• GenBank Accession Number: BC017059
• Gene Symbol: IFI16
• Gene ID (NCBI): 3428
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IFI16 is a member of a family of interferon-inducible nuclear proteins involved in transcriptional regulation functions as a transcriptional repressor. IFI16 may be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. It has been reported that IFI16 participates in the regulation of autophagy, TP53-mediated cell death and innate immune response.