IDS Recombinant antibody

IDS Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242054A7

Cat no : 84623-6-RR

Synonyms

Iduronate 2-sulfatase 42 kDa chain, Iduronate 2-sulfatase 14 kDa chain, Iduronate 2-sulfatase, EC:3.1.6.13, Alpha-L-iduronate sulfate sulfatase

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Validation Data Gallery

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  WB analysis using 84623-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84623-6-RR (IDS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84623-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84623-6-RR against Human IDS were performed. The affinity constant is 11.8 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84623-6-RR targets IDS in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IDS fusion protein Ag31132
• Full Name: iduronate 2-sulfatase
• Calculated Molecular Weight: 312aa,35 kDa; 550aa,62 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC006170
• Gene Symbol: IDS
• Gene ID (NCBI): 3423
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Iduronate 2-sulfatase (IDS) is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations causing IDS deficiency in humans result in the lysosomal storage of these glycosaminoglycans and Hunter syndrome, an X chromosome-linked disease. IDS is synthesized as two precursor forms of 76 and 90 kDa that are converted, through a 62 kDa intermediate, to mature polypeptides due to an internal proteolytic cleavage (PMID: 10838181).