IDO1 Recombinant antibody, PBS Only

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  WB analysis using 84063-3-RR (same clone as 84063-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84063-3-RR (IDO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84063-3-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84063-3-RR (same clone as 84063-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84063-3-RR (IDO1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84063-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84063-3-RR (same clone as 84063-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84063-3-RR (IDO1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84063-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84063-3-RR (same clone as 84063-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84063-3-RR (IDO1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84063-3-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84063-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84063-3-RR against Human IDO1 were performed. The affinity constant is below 1 pM.

IDO1 Uni-rAb^TM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Cat No. 84063-3-PBS

Clone No.241110F2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

241110F2, EC:1.13.11.52, IDO, IDO 1, IDO-1

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Product Information

84063-3-PBS targets IDO1 in WB, IHC, Cytometric bead array, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IDO1 fusion protein Ag3953
• Full Name: indoleamine 2,3-dioxygenase 1
• Calculated Molecular Weight: 403 aa, 45 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC027882
• Gene Symbol: IDO1
• Gene ID (NCBI): 3620
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P14902
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral infections including HIV, HBV, HCV or influenza and also of major bacteria infections, such as Tb, CAP, listeriosis and sepsis. Pathogens are able to highjack the immunosuppressive effects of IDO1 and make use of them to facilitate their own life cycle. MW of IDO1 is 40-42kd (PMID: 14502282; 17055065).