Anti-Human ICAM3/CD50 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using 98097-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human ICAM3/CD50 Rabbit Recombinant Antibody (98097-1-RR, Clone:241418E8) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98097-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human ICAM3/CD50 Rabbit Recombinant Antibody (98097-1-RR, Clone:241418E8) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98097-1-RR

  1x10^6 FC receptor blocked human PBMCs were surface stained with 0.25 ug Anti-Human ICAM3/CD50 Rabbit Recombinant Antibody (98097-1-RR, Clone:241418E8) and PE-conjugated Goat Anti-Rabbit IgG, and 0.25 ug Isotype Control. Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98097-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98097-1-RR against Human ICAM3/CD50 were performed. The affinity constant is 0.366 nM.

ICAM3/CD50 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98097-1-RR

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CDW50, ICAM3, ICAM R, ICAM 3, CD50

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Tested Applications

• Positive FC detected in: FC receptor blocked human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98097-1-RR targets ICAM3/CD50 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: intercellular adhesion molecule 3
• Calculated Molecular Weight: 547 aa, 60 kDa
• GenBank Accession Number: BC058903
• Gene Symbol: ICAM3/CD50
• Gene ID (NCBI): 3385
• RRID: AB_3672244
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P32942
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

The intercellular adhesion molecule (ICAM) family is a subfamily of immunoglobulin superfamily with five known ICAM family members (ICAM1 to ICAM5) which play a role in inflammation, immune responses, and intracellular signaling (PMID: 23287428; 29477378). ICAM3, also known as CD50, is a type I transmembrane glycoprotein that contains five C2-type immunoglobulin-like domains in its extracellular region, a single transmembrane segment, and a C-terminus cytoplasmic tail. ICAM3 is selectively expressed on leukocytes (PMID: 1730916; 8132828). It is the primary ligand for LFA-1 and is involved in various immune processes, including T-cell activation, and macrophage recognition of apoptotic cells (PMID: 11812993; 23775590).