Anti-Human ICAM3/CD50 Rabbit Recombinant Antibody, PBS Only

ICAM3/CD50 Uni-rAb^TM Recombinant Antibody for FC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241418E8

Cat no : 98097-1-PBS

Synonyms

CDW50, ICAM3, ICAM R, ICAM 3, CD50

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Validation Data Gallery

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  FC experiment of human PBMCs using 98097-1-RR (same clone as 98097-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human ICAM3/CD50 Rabbit Recombinant Antibody (98097-1-RR, Clone:241418E8) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98097-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98097-1-RR (same clone as 98097-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human ICAM3/CD50 Rabbit Recombinant Antibody (98097-1-RR, Clone:241418E8) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98097-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98097-1-RR (same clone as 98097-1-PBS)

  1x10^6 FC receptor blocked human PBMCs were surface stained with 0.25 ug Anti-Human ICAM3/CD50 Rabbit Recombinant Antibody (98097-1-RR, Clone:241418E8) and PE-conjugated Goat Anti-Rabbit IgG, and 0.25 ug Isotype Control. Cells were not fixed. This data was developed using the same antibody clone with 98097-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01223-1

  Cytometric bead array standard curve of MP01223-1, ICAM3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 98097-1-PBS. Detection antibody: 84335-3-PBS. Standard: RP02342. Range: 0.781-50 ng/mL.

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  Affinity and Kinetic Characterization of 98097-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98097-1-RR against Human ICAM3/CD50 were performed. The affinity constant is 0.366 nM.

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Product Information

98097-1-PBS targets ICAM3/CD50 as part of a matched antibody pair:

MP01223-1: 98097-1-PBS capture and 84335-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: intercellular adhesion molecule 3
• Calculated Molecular Weight: 547 aa, 60 kDa
• GenBank Accession Number: BC058903
• Gene Symbol: ICAM3/CD50
• Gene ID (NCBI): 3385
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.