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ICAM3/CD50 Recombinant antibody, PBS Only (Detector)

ICAM3/CD50 Uni-rAbTM Recombinant Antibody for IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241418C10

Cat no : 84335-3-PBS

Synonyms

CDW50, ICAM3, ICAM R, ICAM 3, CD50

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Ramos cells using ICAM3/CD50 Recombinant antibody (84335-3-RR)

    IF Staining of Ramos using 84335-3-RR (same clone as 84335-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Ramos cells using ICAM3 antibody (84335-3-RR, Clone: 241418C10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84335-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01223-1

    Cytometric bead array standard curve of MP01223-1

    Cytometric bead array standard curve of MP01223-1, ICAM3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 98097-1-PBS. Detection antibody: 84335-3-PBS. Standard: RP02342. Range: 0.781-50 ng/mL.

Product Information

84335-3-PBS targets ICAM3/CD50 as part of a matched antibody pair:

MP01223-1: 98097-1-PBS capture and 84335-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein
Full Name intercellular adhesion molecule 3
Calculated Molecular Weight 547 aa, 60 kDa
GenBank Accession NumberBC058903
Gene Symbol ICAM3/CD50
Gene ID (NCBI) 3385
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Intercellular Adhesion Molecule 3 (ICAM-3), also known as CD50, is a member of the immunoglobulin superfamily. It is a transmembrane glycoprotein that contains an N-terminus extracellular domain, a single transmembrane domain, and a C-terminus cytoplasmic domain. ICAM-3 possesses 2-9 Ig-like C2-type domains in its extracellular region and is known to bind to the leukocyte adhesion protein LFA-1 . This molecule is expressed on leukocytes and epidermal Langerhans cells and plays a significant role in immune cell interactions and T lymphocyte activation.