ICAM-2/CD102 Recombinant antibody, PBS Only (Capture)

ICAM-2/CD102 Uni-rAb^TM Recombinant Antibody for IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

242237F8

Cat no : 84857-2-PBS

Synonyms

CD102, ICAM2, ICAM-2, ICAM 2, 242237F8

---

Validation Data Gallery

• 

  Sandwich ELISA standard curve of MP01646-1

  Sandwich ELISA standard curve of MP01646-1, Human ICAM2/CD102 Recombinant Matched Antibody Pair - PBS only. 84857-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2002. 84857-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

• 

  Sample test of MP01646-1

  Urine of four individual healthy human donors was measured. The ICAM2/CD102 concentration of detected samples was determined to be 14.2 ng/mL with a range of 6.9-18.8 ng/mL.

• 

  Sample test of MP01646-1

  Serum of four individual healthy human donors was measured. The ICAM2/CD102 concentration of samples was determined to be 425.2 ng/mL with a range of 346.3-500.1 ng/mL.

• 

  IF Staining of HUVEC using 84857-2-RR (same clone as 84857-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using ICAM-2/CD102 antibody (84857-2-RR, Clone: 242237F8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84857-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01646-1

  Cytometric bead array standard curve of MP01646-1, ICAM2/CD102 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84857-2-PBS. Detection antibody: 84857-1-PBS. Standard: Eg2002. Range: 0.625-80 ng/mL.

---

Product Information

84857-2-PBS targets ICAM-2/CD102 as part of a matched antibody pair:

MP01646-1: 84857-2-PBS capture and 84857-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: intercellular adhesion molecule 2
• Calculated Molecular Weight: 31kDa
• GenBank Accession Number: NM_001099789.2
• Gene Symbol: ICAM2/CD102
• Gene ID (NCBI): 3384
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. ICAM2 has six N-linked glycosylation sites at amino acids (asparagines) 47, 82, 105, 153, 178 and 187. This antibody got 55-80 kDa in western blotting maybe due to glycosylation.