FITC-conjugated ICAM-1/CD54 Monoclonal antibody

• Featured Product
• KD/KO Validated

ICAM-1/CD54 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF-P

Conjugate

FITC Fluorescent Dye

Publications

1

CloneNo.

2F9A8

Cat no : FITC-60299

Synonyms

CD54, ICAM-1, ICAM1, ICAM 1, BB2

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Validation Data Gallery

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  IF Staining of human lung cancer using FITC-60299

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using FITC-60299 (ICAM-1 antibody) at dilution of 1:50.

• 

  IF Staining of human lung cancer using FITC-60299

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using FITC-60299 (ICAM-1 antibody) at dilution of 1:50.

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Tested Applications

• Positive IF-P detected in: human lung cancer tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

FITC-60299 targets ICAM-1/CD54 in IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ICAM-1/CD54 fusion protein Ag8309
• Full Name: intercellular adhesion molecule 1
• Calculated Molecular Weight: 90 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC015969
• Gene Symbol: ICAM-1
• Gene ID (NCBI): 3383
• RRID: AB_2883738
• Conjugate: FITC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 498 nm / 526 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784). This antibody is FITC conjugated ICAM1 mouse monoclonal antibody (600299-1-Ig) which has passed in WB, IHC and FC detection.

Publications

Species	Application	Title
	IF	Appl Bionics Biomech; Exosomes Derived from SW480-Resistant Colon Cancer Cells Are Promote Angiogenesis via BMP-2/Smad5 Signaling Pathway.; Authors - Song Yang