CoraLite® Plus 647-conjugated IBA1 Recombinant antibody

IBA1 Uni-rAb^TM Recombinant Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Publications

1

CloneNo.

4D5

Cat no : CL647-81728

Synonyms

AIF 1, AIF1, G1, IBA1, IRT 1, Protein G1

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Validation Data Gallery

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  IF Staining of rat brain using CL647-81728

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite® Plus 647 IBA1 antibody (CL647-81728, Clone: 4D5 ) at dilution of 1:200.

• 

  IF Staining of rat brain using CL647-81728

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite® Plus 647 IBA1 antibody (CL647-81728, Clone: 4D5 ) at dilution of 1:200.

• 

  FC experiment of THP-1 using CL647-81728

  1X10^6 THP-1 cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human IBA1 (CL647-81728, Clone:4D5) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF-P detected in: rat brain tissue
• Positive FC (Intra) detected in: THP-1 cells
• Positive FC detected in: THP-1 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL647-81728 targets IBA1 in IF-P, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IBA1 fusion protein Ag1363
• Full Name: allograft inflammatory factor 1
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC009474
• Gene Symbol: IBA1
• Gene ID (NCBI): 199
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.

Publications

Species	Application	Title
mouse	IF	Nat Commun; SOLID: minimizing tissue distortion for brain-wide profiling of diverse architectures; Authors - Jingtan Zhu