IA-2/PTPRN Monoclonal antibody, PBS Only

IA-2/PTPRN Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D3F5

Cat no : 66045-1-PBS

Synonyms

FLJ16131, IA 2, IA 2/PTP, IA2, IA-2, ICA 512, ICA3, ICA512, Islet cell antigen 512, Islet cell autoantigen 3, PTP IA 2, PTPRN, R PTP N

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Validation Data Gallery

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  WB analysis of human brain using 66045-1-Ig (same clone as 66045-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66045-1-Ig (IA-2/PTPRN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66045-1-Ig (same clone as 66045-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66045-1-Ig (IA-2/PTPRN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66045-1-Ig (same clone as 66045-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66045-1-Ig (same clone as 66045-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66045-1-Ig (same clone as 66045-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66045-1-Ig (same clone as 66045-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66045-1-Ig (same clone as 66045-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66045-1-Ig (same clone as 66045-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66045-1-Ig (same clone as 66045-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 66045-1-Ig (IA-2/PTPRN antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

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Product Information

66045-1-PBS targets IA-2/PTPRN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: IA-2/PTPRN fusion protein Ag17909
• Full Name: protein tyrosine phosphatase, receptor type, N
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 60-65 kDa, 106-115 kDa
• GenBank Accession Number: BC007713
• Gene Symbol: IA-2/PTPRN
• Gene ID (NCBI): 5798
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IA-2/PTPRN is a member of the protein tyrosine phosphatase (PTP) family, which contains a transmembrane region, an intracellular PTP-like domain and an extracellular N-terminus. Experiments found that IA-2/PTPRN localizes to secretory granules and is exclusively expressed in neuroendocrine cells (including pancreatic islets cell). IA-2/PTPRNwas found to be a major autoantigen in insulin-dependent diabetes mellitus and the detection of autoantibodies against IA-2/PTPRN is commonly used as a diabetes diagnosis marker.