Filter: AllWBIHCIF/ICC

WB analysis using 66045-1-Ig (same clone as 66045-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66045-1-Ig (IA-2/PTPRN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 66045-1-Ig (same clone as 66045-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 66045-1-Ig (IA-2/PTPRN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 66045-1-Ig (same clone as 66045-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 66045-1-Ig (IA-2/PTPRN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 66045-1-Ig (same clone as 66045-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 66045-1-Ig (same clone as 66045-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 66045-1-Ig (same clone as 66045-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 66045-1-Ig (same clone as 66045-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 66045-1-Ig (same clone as 66045-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 66045-1-Ig (same clone as 66045-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 66045-1-Ig (same clone as 66045-1-PBS)
Immunofluorescent analysis of HepG2 cells using 66045-1-Ig (IA-2/PTPRN antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

IA-2/PTPRN Monoclonal antibody, PBS Only

IA-2/PTPRN Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66045-1-PBS

Clone No.1D3F5

Review Product

Host / Isotype

Mouse / IgG3

Reactivity

human, pig, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

IA-2, PTPRN, 1D3F5, ICA512-transmembrane fragment, ICA512-N-terminal fragment

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66045-1-PBS targets IA-2/PTPRN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig, rabbit samples.

Tested Reactivity	human, pig, rabbit
Host / Isotype	Mouse / IgG3
Class	Monoclonal
Type	Antibody
Immunogen	IA-2/PTPRN fusion protein Ag17909 Predict reactive species
Full Name	protein tyrosine phosphatase, receptor type, N
Calculated Molecular Weight	105 kDa
Observed Molecular Weight	60-65 kDa, 106-115 kDa
GenBank Accession Number	BC007713
Gene Symbol	IA-2/PTPRN
Gene ID (NCBI)	5798
RRID	AB_11064603
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q16849
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

IA-2/PTPRN is a member of the protein tyrosine phosphatase (PTP) family, which contains a transmembrane region, an intracellular PTP-like domain and an extracellular N-terminus. Experiments found that IA-2/PTPRN localizes to secretory granules and is exclusively expressed in neuroendocrine cells (including pancreatic islets cell). IA-2/PTPRNwas found to be a major autoantigen in insulin-dependent diabetes mellitus and the detection of autoantibodies against IA-2/PTPRN is commonly used as a diabetes diagnosis marker.