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IA-2/PTPRN Monoclonal antibody

IA-2/PTPRN Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D3F5

Cat no : 66045-1-Ig

Synonyms

IA 2/PTP, IA2, IA-2, ICA 512, ICA3, ICA512, Islet cell antigen 512, Islet cell autoantigen 3, PTP IA 2, PTPRN, R PTP N

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using IA-2/PTPRN Monoclonal antibody (66045-1-Ig)

    WB analysis of human brain using 66045-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66045-1-Ig (IA-2/PTPRN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using IA-2/PTPRN Monoclonal antibody (66045-1-Ig)

    WB analysis of human brain using 66045-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66045-1-Ig (IA-2/PTPRN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using IA-2/PTPRN Monoclonal antibody (66045-1-Ig)

    IHC staining of human pancreas cancer using 66045-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using IA-2/PTPRN Monoclonal antibody (66045-1-Ig)

    IHC staining of human pancreas cancer using 66045-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using IA-2/PTPRN Monoclonal antibody (66045-1-Ig)

    IHC staining of human brain using 66045-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using IA-2/PTPRN Monoclonal antibody (66045-1-Ig)

    IHC staining of human brain using 66045-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using IA-2/PTPRN Monoclonal antibody (66045-1-Ig)

    IHC staining of human brain using 66045-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using IA-2/PTPRN Monoclonal antibody (66045-1-Ig)

    IHC staining of human brain using 66045-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IA-2/PTPRN Monoclonal antibody (66045-1-Ig)

    IF Staining of HepG2 using 66045-1-Ig

    Immunofluorescent analysis of HepG2 cells using 66045-1-Ig (IA-2/PTPRN antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

Tested Applications

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman pancreas cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66045-1-Ig targets IA-2/PTPRN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen IA-2/PTPRN fusion protein Ag17909
Full Name protein tyrosine phosphatase, receptor type, N
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 60-65 kDa, 106-115 kDa
GenBank Accession NumberBC007713
Gene Symbol IA-2/PTPRN
Gene ID (NCBI) 5798
RRIDAB_11064603
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IA-2/PTPRN is a member of the protein tyrosine phosphatase (PTP) family, which contains a transmembrane region, an intracellular PTP-like domain and an extracellular N-terminus. Experiments found that IA-2/PTPRN localizes to secretory granules and is exclusively expressed in neuroendocrine cells (including pancreatic islets cell). IA-2/PTPRNwas found to be a major autoantigen in insulin-dependent diabetes mellitus and the detection of autoantibodies against IA-2/PTPRN is commonly used as a diabetes diagnosis marker.

Protocols

Product Specific Protocols
WB protocol for IA-2/PTPRN antibody 66045-1-IgDownload protocol
IHC protocol for IA-2/PTPRN antibody 66045-1-IgDownload protocol
IF protocol for IA-2/PTPRN antibody 66045-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols