PGF Monoclonal Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Mouse / IgG1

Detection: Mouse / IgG2b

Reactivity

Capture: Human

Detection: Human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 31.25-2000 pg/mL

Cat No : MP50063-1

Print datasheet

Synonyms

D12S1900, PGF, PGFL, Placenta growth factor, placental growth factor, PLGF, PlGF 2, SHGC 10760

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP50063-1

  Sandwich ELISA standard curve of MP50063-1, PGF Monoclonal Matched Antibody Pair - PBS only. 68644-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Eg0653. 68644-1-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL.

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Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP50063-1 targets PGF in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 31.25-2000 pg/mL (Sandwich ELISA);

Capture antibody: PGF Monoclonal antibody, PBS Only (Capture) 68644-2-PBS (4F4F3). 100 μg. Concentration 1 mgl/ml.

Detection antibody: PGF Monoclonal antibody, PBS Only (Detector) 68644-1-PBS (3E9D2). 100 μg. Concentration 1 mgl/ml.

Alternative PGF matched antibody pairs: MP00146-1, MP00146-2, MP00146-3, MP50063-2

Matched pair protein standard: Eg0653

Unconjugated mouse monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

• Capture antibody
• Detection antibody

• Catalog Number: 68644-2-PBS
• Tested Reactivity: Human
• Immunogen: Recombinant protein
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Affinity:
  K_D=1.12 x 10^-10M
  K_Off=2.03 x 10^-6M
  K_On=1.81 x 10^4M
• Full Name: placental growth factor
• Synonyms: D12S1900, PGF, PGFL, Placenta growth factor, placental growth factor, PLGF, PlGF 2, SHGC 10760
• GenBank accession number: NP_001193941.1
• Gene symbol: PGF
• Uniprot: P49763-2
• Gene ID (NCBI): 5228
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

• Catalog Number: 68644-1-PBS
• Tested Reactivity: Human
• Immunogen: Recombinant protein
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Affinity:
  K_D=2.49 x 10^-9M
  K_Off=1.28 x 10^-4M
  K_On=5.12 x 10^4M
• Full Name: placental growth factor
• Synonyms: D12S1900, PGF, PGFL, Placenta growth factor, placental growth factor, PLGF, PlGF 2, SHGC 10760
• GenBank accession number: NP_001193941.1
• Gene symbol: PGF
• Uniprot: P49763-2
• Gene ID (NCBI): 5228
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.