PGF Monoclonal antibody, PBS Only (Capture)

PGF Monoclonal Antibody for Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4F4F3

Cat no : 68644-2-PBS

Synonyms

D12S1900, PGF, PGFL, Placenta growth factor, placental growth factor, PLGF, PlGF 2, SHGC 10760

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP50063-1

  Sandwich ELISA standard curve of MP50063-1, PGF Monoclonal Matched Antibody Pair - PBS only. 68644-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Eg0653. 68644-1-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68644-2-PBS targets PGF as part of a matched antibody pair:

MP50063-1: 68644-2-PBS capture and 68644-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Affinity:
  K_D=1.12 x 10^-10M
  K_Off=2.03 x 10^-6M
  K_On=1.81 x 10^4M
• Immunogen: Recombinant protein
• Full Name: placental growth factor
• Calculated Molecular Weight: 17KD
• GenBank Accession Number: NP_001193941.1
• Gene Symbol: PGF
• Gene ID (NCBI): 5228
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.