Human IgG Heavy chain Monoclonal antibody, PBS Only

Human IgG Heavy chain Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1C6B4

Cat no : 67760-1-PBS

Synonyms

human IgG, Human IgG Heavy chain

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Validation Data Gallery

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  WB analysis of human plasma using 67760-1-Ig (same clone as 67760-1-PBS)

  16 nL human plasma were subjected to SDS PAGE followed by western blot with 67760-1-Ig (Human IgG Heavy chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67760-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67760-1-Ig (same clone as 67760-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67760-1-Ig (Human IgG Heavy chain antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67760-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 67760-1-Ig (same clone as 67760-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67760-1-Ig (Human IgG Heavy chain antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67760-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 67760-1-Ig (same clone as 67760-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67760-1-Ig (Human IgG Heavy chain antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67760-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 67760-1-Ig (same clone as 67760-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Human IgG Heavy chain antibody (67760-1-Ig, Clone: 1C6B4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67760-1-PBS in a different storage buffer formulation.

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Product Information

67760-1-PBS targets Human IgG Heavy chain in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: nature protein
• Full Name: Human IgG Heavy chain
• Calculated Molecular Weight: 155 kDa
• Observed Molecular Weight: ~50 kDa
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

This antibody detects the heavy chain of human IgG.