IgG Heavy chain Monoclonal antibody

Human IgG Heavy chain Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1C6B4

Cat no : 67760-1-Ig

Synonyms

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Validation Data Gallery

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  WB analysis of human plasma using 67760-1-Ig

  16 nL human plasma were subjected to SDS PAGE followed by western blot with 67760-1-Ig (Human IgG Heavy chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 67760-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67760-1-Ig (Human IgG Heavy chain antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67760-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67760-1-Ig (Human IgG Heavy chain antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67760-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67760-1-Ig (Human IgG Heavy chain antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 67760-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Human IgG Heavy chain antibody (67760-1-Ig, Clone: 1C6B4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: human plasma
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67760-1-Ig targets Human IgG Heavy chain in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: nature protein
• Full Name: Human IgG Heavy chain
• Calculated Molecular Weight: 155 kDa
• Observed Molecular Weight: ~50 kDa
• RRID: AB_2918528
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody detects the heavy chain of human IgG.