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Di/Tri-Methyl-Histone H3 (Lys36) Recombinant antibody, PBS Only

Di/Tri-Methyl-Histone H3 (Lys36) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Dot Blot, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Dot Blot, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241139G6

Cat no : 84329-1-PBS

Synonyms

HIST1H3A, 241139G6, Histone-H3, HistoneH3, Histone H3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Di/Tri-Methyl-Histone H3 (Lys36) Recombinant antib (84329-1-RR)

    WB analysis using 84329-1-RR (same clone as 84329-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84329-1-RR (Di/Tri-Methyl-Histone H3 (Lys36) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84329-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Di/Tri-Methyl-Histone H3 (Lys36) Recombinant antib (84329-1-RR)

    IF Staining of HeLa using 84329-1-RR (same clone as 84329-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84329-1-RR, Clone: 241139G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84329-1-PBS in a different storage buffer formulation.

  • Dot Blot experiment of peptide using Di/Tri-Methyl-Histone H3 (Lys36) Recombinant antib (84329-1-RR)

    Dot Blot experiment of peptide using 84329-1-RR (same clone as 84329-1-PBS)

    Dot blot analysis was used to confirm the specificity of Di/Tri-Methyl-Histone H3 (Lys36) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 84329-1-PBS in a different storage buffer formulation.

Product Information

84329-1-PBS targets Di/Tri-Methyl-Histone H3 (Lys36) in WB, IF/ICC, Dot Blot, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name histone cluster 1, H3a
Observed Molecular Weight15 kDa
GenBank Accession NumberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.