Di/Tri-Methyl-Histone H3 (Lys36) Recombinant antibody

Di/Tri-Methyl-Histone H3 (Lys36) Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Dot Blot, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Dot Blot, ELISA

Conjugate

Unconjugated

CloneNo.

241139G6

Cat no : 84329-1-RR

Synonyms

HIST1H3A, 241139G6, Histone-H3, HistoneH3, Histone H3

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Validation Data Gallery

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  WB analysis using 84329-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84329-1-RR (Di/Tri-Methyl-Histone H3 (Lys36) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84329-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84329-1-RR, Clone: 241139G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Dot Blot experiment of peptide using 84329-1-RR

  Dot blot analysis was used to confirm the specificity of Di/Tri-Methyl-Histone H3 (Lys36) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, U-87 MG cells, U-251 cells, NIH/3T3 cells, mouse liver tissue, mouse brain tissue
• Positive IF/ICC detected in: HeLa cells
• Positive Dot Blot detected in: peptide

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• DOT BLOT: DOT BLOT : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84329-1-RR targets Di/Tri-Methyl-Histone H3 (Lys36) in WB, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: histone cluster 1, H3a
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC066245
• Gene Symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.