ChromoTek His Fab-Trap® Magnetic Particles M-270

The ChromoTek His Fab-Trap® Magnetic Particles M-270 consists of an anti-His-tag Fab fragment, which is coupled to magnetic particles. It can be used for the immunoprecipitation of proteins fused to the His-tag from cell extracts of various organisms. It is highly recommended when very large proteins/complexes are being investigated.

Specificity

6*His, His-Tag (sequence: HHHHHH)

Applications

IP, Co-IP

Type

Fab-fragment

Conjugate

Magnetic Particles M-270

Cat no : hfd

Synonyms

His-trap, 6*His Fab-Trap, His-tag Fab-Trap

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Validation Data Gallery

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  IP using His Fab-Trap® Magnetic Particles M-270 (hfd) from untransfected HEK293T (mock) and HEK293T cells transfected with either His-TOM70 or TOM70-His fusion proteins. WB detection of TOM70 protein was also done on samples from the Input (I), Flow-Through (F), and Bound (B) fractions using Anti-TOM70 Polyclonal Antibody (14528-1-AP) and ChromoTek Nano-Secondary alpaca anti-rabbit IgG, CoraLite Plus 488 (srb2GCL488-1).

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Product Information

The ChromoTek His Fab-Trap® Magnetic Particles M-270 consists of an anti-His-tag Fab fragment, which is coupled to magnetic particles. It can be used for the immunoprecipitation of proteins fused to the His-tag from cell extracts of various organisms. It is highly recommended when very large proteins/complexes are being investigated.

• Description: Immunoprecipitation of His-tagged proteins with anti-His-tag Fab-fragment conjugated to magnetic particles.;

  • Fast, reliable & efficient one-step immunoprecipitation;

  • Ready-to-use;

  • Low background;

  • Stable under harsh washing conditions;

  • Suitable for downstream mass spec analysis;

  • Works in samples from: mammals, plants, yeast, etc.

• Applications: IP, Co-IP
• Specificity/Target: Binds specifically to the His-tag (also known as hexahistidine or His6-tag) fused to a protein of interest at N-, C- or internal position. Please note that the affinity is highest for a C-terminal fusion (dissociation constant KD of 10 nM for a C-terminal His-tag and 220 nM for an N-terminal His-tag).
• Binding Capacity: 1 μg of recombinant His-tagged protein (~30 kDa) per 25 μL bead slurry
• Conjugate: Magnetic Particles M-270; size: 2.8 µM
• Elution buffer: 2x SDS-sample buffer (Lämmli), 200 mM glycine pH 2.5 or 100 µM His-Peptide
• Wash Buffer Compatibility: 2 M NaCl, 5 mM DTT, 5 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, <0.1% SDS, 3-4 M Urea
• Type: Fab-fragment
• Class: Recombinant
• Host: Mouse
• Affinity (KD): 10 nM for a C-terminal His-tag and 220 nM for a N-terminal His-tag
• Compatibility with mass spectrometry: The His Fab-Trap® is optimized for on-bead digestion. For the application note, please click here: ; On-bead digest protocol for mass spectrometry
• RRID: AB_3665061
• Storage Buffer: PBS pH 7.4. Preservative: 0.09% sodium azide
• Storage Condition: Shipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. Do not freeze!

Other Formats

His Fab-Trap® Magnetic Particles M-270 Kit