CoraLite® Plus 488-conjugated Hexokinase 2 Monoclonal antibody

Hexokinase 2 Monoclonal Antibody for WB, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A11C3

Cat no : CL488-66974

Synonyms

HK2, Hexokinase-B, Hexokinase-2, hexokinase2, Hexokinase type II



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66974 targets Hexokinase 2 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Hexokinase 2 fusion protein Ag16895
Full Name hexokinase 2
Calculated Molecular Weight 102 kDa
Observed Molecular Weight 102 kDa
GenBank Accession NumberBC021116
Gene Symbol Hexokinase 2
Gene ID (NCBI) 3099
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236)

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 Hexokinase 2 antibody CL488-66974Download protocol
Standard Protocols
Click here to view our Standard Protocols