Hexokinase 2 Monoclonal antibody, PBS Only (Detector)

Hexokinase 2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A11C3

Cat no : 66974-1-PBS

Synonyms

HK2, HK 2, Hexokinase-B, Hexokinase-2, hexokinase2

---

Validation Data Gallery

• 

  WB analysis using 66974-1-Ig (same clone as 66974-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66974-1-Ig (Hexokinase 2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66974-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 66974-1-Ig (same clone as 66974-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66974-1-Ig (Hexokinase 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66974-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66974-1-Ig (same clone as 66974-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Hexokinase 2 antibody (66974-1-Ig, Clone: 2A11C3 ) at dilution of 1:0 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66974-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 66974-1-Ig (same clone as 66974-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Hexokinase 2 Monoclonal antibody (66974-1-Ig, Clone:2A11C3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66974-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50448-3

  Cytometric bead array standard curve of MP50448-3, HK2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66974-4-PBS. Detection antibody: 66974-1-PBS. Standard:Ag16895. Range: 0.781-100 ng/mL.

---

Product Information

66974-1-PBS targets Hexokinase 2 as part of a matched antibody pair:

MP50448-3: 66974-4-PBS capture and 66974-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Hexokinase 2 fusion protein Ag16895
• Full Name: hexokinase 2
• Calculated Molecular Weight: 102 kDa
• Observed Molecular Weight: 102 kDa
• GenBank Accession Number: BC021116
• Gene Symbol: Hexokinase 2
• Gene ID (NCBI): 3099
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236). The antibody also recognizes Hexokinase 1.