Hexokinase 1 Monoclonal antibody, PBS Only

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  WB analysis using 68419-1-Ig (same clone as 68419-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68419-1-Ig (HK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68419-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 68419-1-Ig (same clone as 68419-1-PBS)

  IP result of anti-Hexokinase 1 (IP:68419-1-Ig, 4ug; Detection:68419-1-Ig 1:2000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 68419-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68419-1-Ig (same clone as 68419-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68419-1-Ig (Hexokinase 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68419-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68419-1-Ig (same clone as 68419-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Hexokinase 1 antibody (68419-1-Ig, Clone: 2B4F9 ) at dilution of 1:2049 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004). This data was developed using the same antibody clone with 68419-1-PBS in a different storage buffer formulation.

Hexokinase 1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 68419-1-PBS

Clone No.2B4F9

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

HK1, Hexokinase-1, Hexokinase1, Hexokinase type I, EC:2.7.1.1

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Product Information

68419-1-PBS targets Hexokinase 1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Hexokinase 1 fusion protein Ag8015
• Full Name: hexokinase 1
• Calculated Molecular Weight: 102 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC008730
• Gene Symbol: Hexokinase 1
• Gene ID (NCBI): 3098
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P19367
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

The hexokinases (HKs) catalyze the first obligatory step in glucose metabolism to generate glucose-6-phosphate. This not only furthers glucose entry by maintaining the concentration gradient for facilitated glucose influx but also provides the first intermediate for essentially all major pathways using glucose. There are four conventional HK isoforms, HK1/2/3/4, encoded by four different genes. Most adult tissues express only HK1. Muscle and adipose tissue use HK2 for glycolysis, liver, and pancreatic beta cells express HK4 (also called glucokinase) and do not express HK1 or HK2. (PMID: 31434645, PMID: 31848318)