Hexokinase 1 Monoclonal antibody

Hexokinase 1 Monoclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B4F9

Cat no : 68419-1-Ig

Synonyms

Brain form hexokinase, hexokinase 1, Hexokinase type I, HK I, HK1, HK1 ta, HK1 tb, HK1 tc, HKI, HXK1

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Validation Data Gallery

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  WB analysis using 68419-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68419-1-Ig (HK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 68419-1-Ig

  IP result of anti-Hexokinase 1 (IP:68419-1-Ig, 4ug; Detection:68419-1-Ig 1:2000) with HeLa cells lysate 1560 ug.

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  IHC staining of mouse brain using 68419-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68419-1-Ig (Hexokinase 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HT-1080 cells, U-87 MG cells, SK-BR-3 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, pig brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68419-1-Ig targets Hexokinase 1 in WB, IP, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Hexokinase 1 fusion protein Ag8015
• Full Name: hexokinase 1
• Calculated Molecular Weight: 102 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC008730
• Gene Symbol: HK1
• Gene ID (NCBI): 3098
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The hexokinases (HKs) catalyze the first obligatory step in glucose metabolism to generate glucose-6-phosphate. This not only furthers glucose entry by maintaining the concentration gradient for facilitated glucose influx but also provides the first intermediate for essentially all major pathways using glucose. There are four conventional HK isoforms, HK1/2/3/4, encoded by four different genes. Most adult tissues express only HK1. Muscle and adipose tissue use HK2 for glycolysis, liver, and pancreatic beta cells express HK4 (also called glucokinase) and do not express HK1 or HK2. (PMID: 31434645, PMID: 31848318)