Hemopexin Monoclonal antibody, PBS Only

Hemopexin Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

pig, rat, human

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A9D6

Cat no : 66479-1-PBS

Synonyms

HPX, Beta-1B-glycoprotein, Beta 1B glycoprotein, 3A9D6

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Validation Data Gallery

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  WB analysis of human plasma using 66479-1-Ig (same clone as 66479-1-PBS)

  0.7 μL human plasma was subjected to SDS PAGE followed by western blot with 66479-1-Ig (Hemopexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

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  WB analysis of pig plasma using 66479-1-Ig (same clone as 66479-1-PBS)

  0.7 μL pig plasma was subjected to SDS PAGE followed by western blot with 66479-1-Ig (Hemopexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

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  WB analysis of rat serum using 66479-1-Ig (same clone as 66479-1-PBS)

  0.7 μL rat serum was subjected to SDS PAGE followed by western blot with 66479-1-Ig (Hemopexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 66479-1-Ig (same clone as 66479-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66479-1-Ig (Hemopexin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66479-1-Ig (same clone as 66479-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66479-1-Ig (Hemopexin antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66479-1-Ig (same clone as 66479-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66479-1-Ig (Hemopexin antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66479-1-Ig (same clone as 66479-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66479-1-Ig (Hemopexin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 66479-1-Ig (same clone as 66479-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Hemopexin antibody (66479-1-Ig, Clone: 3A9D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66479-1-Ig (same clone as 66479-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human Hemopexin (66479-1-Ig, Clone:3A9D6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

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Product Information

66479-1-PBS targets Hemopexin in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with pig, rat, human samples.

• Tested Reactivity: pig, rat, human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Hemopexin fusion protein Ag8533
• Full Name: hemopexin
• Calculated Molecular Weight: 254 aa, 29 kDa
• Observed Molecular Weight: 65-75 kDa
• GenBank Accession Number: BC005395
• Gene Symbol: Hemopexin
• Gene ID (NCBI): 3263
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Hemopexin (HPX) is the plasma protein responsible for scavenging heme, thus preventing heme-mediated oxidative stress and heme-bound iron loss. In addition, hemopexin blocks heme activation of immune receptors and vascular inflammatory processes. It is mainly expressed in liver, the synthesis of which is induced after inflammation. Alterations of plasma hemopexin level have been linked to disorders like atherosclerosis and inflammatory diseases.