CoraLite® Plus 488-conjugated IgG4 Monoclonal antibody

Human IgG4 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A1A2

Cat no : CL488-66408

Synonyms

IGHG4

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL488-66408

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66408 (Human of IgG4 antibody) at dilution of 1:100.

• 

  IF Staining of human tonsillitis using CL488-66408

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66408 (Human of IgG4 antibody) at dilution of 1:100.

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Tested Applications

• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66408 targets Human IgG4 in IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: immunoglobulin heavy constant gamma 4 (G4m marker)
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: AL928742
• Gene Symbol: IGHG4
• Gene ID (NCBI): 3503
• RRID: AB_2883328
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

This antibody detects the heavy chain of human IgG4. The immunogen of this antibody is the specific domain "NKGLPSSIEK" of IGHG4.