CoraLite®594-conjugated HVCN1 Monoclonal antibody

HVCN1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E4C4

Cat no : CL594-66449

Synonyms

HV1, HVCN1, VSOP

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL594-66449

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 HVCN1 antibody (CL594-66449, Clone: 1E4C4 ) at dilution of 1:200.

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  IF Staining of human tonsillitis using CL594-66449

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 HVCN1 antibody (CL594-66449, Clone: 1E4C4 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66449 targets HVCN1 in IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HVCN1 fusion protein Ag5350
• Full Name: hydrogen voltage-gated channel 1
• Calculated Molecular Weight: 273 aa, 32 kDa
• Observed Molecular Weight: 28-35 kDa, 40 kDa, 60 kDa
• GenBank Accession Number: BC032672
• Gene Symbol: HVCN1
• Gene ID (NCBI): 84329
• RRID: AB_2919994
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

HVCN1, also named as VSOP and HV1, Belongs to the hydrogen channel family. HVCN1 mediates the voltage-dependent proton permeability of excitable membranes. It forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, HVCN1 is accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis. HVCN1, the voltage-sensitive proton channel, is present in human sperm and is an important regulator of the functional maturation of sperm. HVCN1 has four isoforms with MW 28-32 kDa or 40 kDa (modification). It has a dimer form with MW 60 kDa.